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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Gynecol Oncol. 2022 Mar 12;165(2):239–247. doi: 10.1016/j.ygyno.2022.02.022

Figure 4: Effects of HIPEC with platinum on heat-shock and immune-related protein expression in normal and tumor tissue.

Figure 4:

(A) Heatmap of human heat shock protein antibody array demonstrating changes in protein expression levels of normal and tumor tissue samples after HIPEC with platinum exposure, color-coded according to the legend (left). Following background subtraction, the data were normalized to the positive control signals. For each protein, the mean Log2 fold-change post-HIPEC samples compared to pre-HIPEC samples are shown on the right. Error bars, s.d. of mean.

(B) Gene expression Log2 fold-change normalized by gene expression levels pre-HIPEC exposure (Error bars, s.d. of mean). Genes selected for illustration are those that correspond with the 9 heat shock proteins HSP90, GRP75, HSP27, Ubiquitin+1, HSP32, HSP10, HSP40, HSP60 and HSP70 investigated in the protein antibody array (Table, right).

(C) Western blot analysis of IL-6 protein in normal and tumor samples pre- and post-HIPEC with platinum exposure. Tubulin was used as protein loading control (bottom). Mean of Log2 fold-change of post-HIPEC samples as compared to pre-HIPEC samples (right). Error bars, s.d. of mean. Cases are labeled H1 – H4, for normal and tumor tissues.

(D) ELISA of IL-6 protein in normal and tumor samples pre- and post-HIPEC with platinum exposure. Error bars, s.d. of mean.