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. Author manuscript; available in PMC: 2022 Nov 2.
Published in final edited form as: Clin Cancer Res. 2022 May 2;28(9):1979–1990. doi: 10.1158/1078-0432.CCR-20-0468

Figure 2.

Figure 2.

Modulation of IR-mediated DNA damage signaling by onalespib in patient-derived glioma stem cells. Immunoblot analysis of effect on levels of p-ATM, p-ATR and p-CHK1 (as measures of DNA damage signaling) and cleaved PARP (reflecting induction of apoptosis) in GSC811, GSC11, GSC262, and GSC23 cells after IR treatment (8 Gy) and suppression of this effect by pretreatment with onalespib (0.4μM). GAPDH was loaded as a control and HSP70 assayed as a measure of HSP90 inhibition. Figures are representative of three independent experiments.