Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 3;13:2406. doi: 10.1038/s41467-022-30174-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Real-time PCR analysis for mRNA levels of Mincle in T_H0, T_H1, T_H2, T_H17, and Treg cells after 3 days polarization. The expression levels were normalized to the expression of β-actin, n = 3 biological replicates. b Western analysis of Mincle protein in polarized T_H1, T_H2, T_H17, and Treg cells from WT and Mincle-deficient mice, β-actin as a loading control, data are representative of three independent experiments, density values measured using Image J for the representative blot shown, ND not detected. c Real-time PCR analysis for Mincle mRNA levels in CD4+ cells stimulated with anti-CD3/CD28 in the presence of indicated cytokines for 72 h, n = 3 biological replicates. d Flow cytometry analysis of wild-type and Mincle-deficient T_H1, T_H1, T_H17, and T_reg cells with the indicated antibodies, n = 4 biological replicates. ***P < 0.001 (Two sided student’s t test for a and d, Two-way ANOVA for c) Data are represented as mean ± SD. Exact P values for asterisks (from left to right): a 0.00004, c 0.0015, 0.0007, 0.0089, 0.0056, 0.0086.