Fig. 3. Mincle is required for TH17 mediated EAE progression.
a–c Lymph nodes were harvested from MOG immunized mice on Day 9 post immunization, and cells were cultured with increasing concentrations of MOG for 72 h. Cytokine concentrations in the culture media were measured by ELISA, n = 4 biological replicates. d, e Mean clinical score of EAE mice (n = 5) induced by adoptive transfer of MOG-reactive d TH1 or e TH17 cells. f Brain lymphocytes from TH17 recipient mice were harvested at the peak of disease and analyzed by flow cytometry with indicated antibodies by flow cytometry, n = 5 biological replicates. g H&E staining and Luxol fast blue staining of lumbar spinal cords at peak of the disease in recipient mice. Scale bar, 100 μM. Arrows in the upper panel indicate inflammatory cells infiltration, and arrows in the lower panel indicate demyelination area. Representative data are shown for n = 4. h Percentage of CD45+ CD4+ cells of total CNS infiltrated cells from Rag1−/− mice that received MOG-reactive TH17 cells, n = 4 biological replicates. i Cell numbers of CNS-infiltrating CD4+ T cells from (h, j). Percent of Ki67/CD4 double-positive cells from spinal cords from adoptive transfer experiments 9 days after transfer (representative images shown in Supplemental Fig. 3f.). n = 5, biological replicates. **P < 0.01 (Two-sided student’s t test for a–c, f, h–j). ***P < 0.001 (two-way ANOVA for d, e). Data are represented as mean ± SD. Exact P values for asterisks (from left to right): e <0.0001 f 0.0009 0.0033 0.0037 h 0.0025 i 0.0069 j 0.0015.