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. 2022 May 3;13:2406. doi: 10.1038/s41467-022-30174-1

Fig. 4. Mincle activation promotes TH17 differentiation.

Fig. 4

a Flow cytometric analysis of IL-17A and IFNγ from Mincle f/+Lck-Cre and Mincle f/fLck-Cre TH17 polarized with or without β-glucosylceramide (5 μg/ml) stimulation, n = 4 biological replicates. b CFSE staining of TH17 cells polarized with or without β-glucosylceramide (5 μg/ml). Data are presented as mean fluorescent intensity, n = 4 biological replicates. c Real-time PCR of mRNA of inflammatory genes in TH17 cells polarized with or without β-glucosylceramide (1 μg/ml), n = 3 biological replicates. Expression was normalized to expression of β-actin. *p < 0.05, **p < 0.01 ***p < 0.001 (Two sided student’s t test). Data are represented as mean ± SD. Exact P values for asterisks (from left to right): a <0.0001 0.00016 b <0.0001 < 0.0001 c 0.00005 0.00088 0.0036 0.0172 0.0225 0.0341 0.0207 0.0178 0.0101 0.0128.