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. 2022 May 3;13:2406. doi: 10.1038/s41467-022-30174-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Flow cytometric analysis of IL-17A and IFNγ following T_H17 polarization with lipids extracted from spinal cord of naive, EAE or AMP-DNM treated EAE mice, n = 4 biological replicates. b Measurement of β-glucosylceramide from the spinal cord of naive, EAE and AMP-DNM treated EAE mice, n = 3, 5, 4 biological replicates. c Flow cytometric analysis of IL-17A and IFNγ following T_H17 polarization with lipids extracted from supernatants of oligodendrocytes after the indicated treatments, n = 4 biological replicates. d Measurement of β-glucosylceramide from the oligodendrocyte culture medium under indicated stimulations for 12 h, n = 3 biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001 (Two sided student’s t test). Data are represented as mean ± SD. Exact P values for asterisks (from left to right): a < 0.0001 0.00004 0.0091 b 0.0376 0.0289 0.00047 0.0065 0.01178 0.0313 0.0193 0.00043 0.0030 0.0056 c 0.00013 0.00038 0.00366 d 0.0131 0.0055 0.0005 0.0005 0.0001 0.0393 0.0220 0.0190.