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. 2022 May 3;13:2403. doi: 10.1038/s41467-022-30169-y

Fig. 1. Hypoxia-mediated behavioral changes require reactive oxygen species.

Fig. 1

a C. elegans increase locomotion in response to changes in oxygen concentration. Staged wild-type (N2 Bristol) L4 body bends were scored on unseeded plates in response to changes in oxygen. Baseline was recorded, and worms were then subjected to hypoxia and reoxygenation. Where indicated, worms were grown on plates containing the superoxide dismutase and catalase mimetic EUK134 (100 µM) 24 h prior. Data are mean ± SD. N = 15 independent animals across 3 technical replicates, **p < 0.01 (Two-way ANOVA, Sidak’s multiple comparisons). b, c Complex I ROS increased C. elegans locomotion. Wild-type L4 worms were treated with (b) paraquat (1 mM) or (c) rotenone (1 µM) 24 h prior. Body bends were scored on unseeded plates. Data are mean ± SD. N = 15 independent animals across three technical replicates, **p < 0.01 (One-way ANOVA, Dunnett’s multiple comparisons). Full dose–response in Supplementary Fig. 1.