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. 2022 May 3;13:2403. doi: 10.1038/s41467-022-30169-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic illustration of complex I ROS generation and sites of targeted modulations. b Effects of MnPyP (superoxide dismutase mimetic) and mitoTempo (mTEMPO) on complex I ROS-induced changes in C. elegans locomotion. nuo-1::Supernova were incubated with MnPyP (100 µM) or mTEMPO (10 µM) for 24 h. Worms were then individually singled and transferred into an unseeded plate and body bends were scored for 15 s with and without illumination (MVX, 5.6 mW/mm²). Data are mean ± SD. N = 15 independent animals across 3 technical replicates, **p < 0.01 (Three-way ANOVA, Tukey’s multiple comparisons). Full dose response and wild-type controls available in Supplementary Fig. 10. c Effects of EUK-134 (catalase mimetic, EUK) and NAC on complex I ROS-induced changes in C. elegans locomotion. nuo-1::Supernova were incubated with EUK (100 µM and NAC (2.5 mM) for 24 h. Worms were then individually singled and transferred into an unseeded plate and body bends were scored for 15 s with and without illumination (MVX, 5.6 mW/mm²). Data are mean ± SD. N = 15 independent animals across 3 technical replicates, **p < 0.01 (Three-way ANOVA, Tukey’s multiple comparisons). Full dose response and wild-type controls available in Supplementary Fig. 10. d Complex I ROS-induced changes in C. elegans locomotion requires sod-2/-3. L4 nuo-1::Supernova, sod-2/-3, nuo-1::Supernova + sod-2/3, nuo-1::Supernova + sod-2, and nuo-1::Supernova + sod-3 worms were individually singled and transferred to an unseeded plate. Body bends were then scored for 15 s with and without illumination (MVX, 5.6 mW/mm²). Data are mean ± SD. N = 15 independent animals across 3 technical replicates, **p < 0.01 (Two-way ANOVA, Sidak’s multiple comparisons). e Superoxide dismutase mimetic rescues photolocomotion in the absence of sod-2/-3. nuo-1::Supernova + sod-2/3 worms were incubated with MnPyP (100 µM) for 24 h. Worms were then individually singled and transferred into an unseeded plate and body bends were scored for 15 s with and without illumination (MVX, 5.6 mW/mm²). Data are mean ± SD. N = 15 independent animals across 3 technical replicates, **p < 0.01 (two-way ANOVA, Tukey’s multiple comparisons).