Table 2.
E. coli 16S spectral identification coverage and FDR.
| Dataset | Q-TOFa | Synapta | Orbitrapa |
|---|---|---|---|
| mgf scansb | 1720 | 1107 | 4513 |
| Top targetsc | 771 | 410 | 693 |
| Top decoysc | 36 | 43 | 95 |
| Unique target sequencesd | 141(l) | 111(h) | 135(l) | 107(h) | 160(l) | 153(h) |
| Highest Sp score | 1.517 | 2.267 | 1.946 |
| Unique target sequences (10% FDR)d | 141 (l) | 111(h) | 98 (l) | 106 (h) | 111 (l) | 110 (h) |
| Unique target sequences (5% FDR)d | 121 (l) | 111 (h) | 54 (l) | 96 (h) | 105 (l) | 90 (h) |
| Unique target sequences (1% FDR)d | 100 (l) | 94 (h) | 30 (l) | 56 (h) | 69 (l) | 69 (h) |
a(l) and (h) refer to the number of light- and heavy-labeled RNA fragments identified.
bTotal number of acquired MS2 spectra in the input file.
cTop targets and decoys refer to rank 1 OSMs.
dTotal number of targets with unique sequences. Light- and heavy- identifications are counted separately. FDR threshold (if applied) is specified in the parenthesis.