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. 2022 May 3;13:2424. doi: 10.1038/s41467-022-30057-5

Table 3.

E. coli 16S rRNA methylations identified in the Q-TOF dataset.

Mod Position Target sequence Sp (l)a ΔSpDb (l)a Sp (h)a ΔSpDb (h)a Min FDRc
[m7G] 527 525-CC[m7G]CG-529 0.299 0.55 0.231 0.29 3%
[m5C] 967 967-[m5C]AACG-971 0.711 0.86 0.752 0.86 <1%
[m4Cm] 1402 1402-[m4Cm]CCG-1405 0.833 0.82 1.009 0.83 <1%
[m5C] 1407 1406-U[m5C]ACACCAUG-1415 0.445 0.91 0.888 0.74 <1%
[m3U] 1498 1498-[m3U]AACAAG-1504 0.993 0.67 1.188 0.94 <1%
[m26A] 1518 1518-[m26A][m26A]CCUG-1523 1.445 0.75 1.429 0.74 <1%
[m26A] 1519 1518-[m26A][m26A]CCUG-1523 1.445 0.75 1.429 0.74 <1%

aData shown for light- (l) or heavy- (h) labeled RNA species identified.

bΔSpD is defined as ΔSp score of the highest-scoring competing decoy.

cLowest FDR threshold at which the modification is detected.