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. 2022 May 3;7:145. doi: 10.1038/s41392-022-00990-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — High-resolution characterization of tumor microenvironment (TME) by single-cell sequencing. Cellular architecture of the TME-infiltrated immune cells in the TME is broadly grouped using flow cytometry-based markers. Single-cell sequencing has made it possible to characterize the phenotypic heterogeneity of immune cells at the transcriptomic, proteomic, and epigenetic levels