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. 2022 May 3;13:2422. doi: 10.1038/s41467-022-30101-4

Fig. 1. Dynamin oligomerization defines cell stiffness by influencing the actin architecture in renal epithelial cells.

Fig. 1

a Representative SEM images of MDCK cells treated with DMSO (0.1%) or Bis-T-23 (30 µM, 0.1% DMSO) for 10 min prior to the addition of DMSO (0.1%) or LatA (0.2 µM, 0.1% DMSO) for 20 min. Enlarged images of the insets (orange boxed regions) show the arrangement, distribution, and density of microvilli at the apical membrane. b Representative PR-EM images of MDCK cells treated as explained in (a). The images show changes in the organization of the actomyosin cortex in MDCK cells under the indicated conditions. c Representative images of Young’s Modulus maps of MDCK cells treated as explained in (a). d, e Bar graphs representing Young’s Modulus depicting cell stiffness measured at the cell–cell junction (d) or at the apical membrane (e). Each symbol represents the average stiffness of a single cell. Results shown in d, e were generated from at least 10 cells from at least three culture dishes. Error bars, mean ± S.D. (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, unpaired two-tailed t-test). ns, not significant.