Figure 2.

The bone-marrow niche is less pro-inflammatory in BU-conditioned NSG mice

(A) Cytokine secretion measured using the mouse 48-plex Luminex assay in the plasma of NSG mice at 2 and 24 h (H) following TBI (2.1 Gy, n = 5) or BU (66 mg/kg, n = 5) conditioning. The 2 and 24 h time points correspond to the time of transplantation after conditioning, respectively. (B) Cytokine secretion in bone marrow 24 h (24H) post-conditioning. (A and B) Fold changes were calculated relative to untreated NSG control mice (n = 4). Statistically significant changes are depicted in bold. No statistically significant cytokine changes were found other than those depicted. (C–F) Frequency and median fluorescence intensity (MFI) monocyte and macrophage progenitors (CD11b+, Ly6C+) and erythrocytes (TER119+) measured in the bone marrow by flow cytometry. (A–F) Statistical analysis: one-way ANOVA with Tukey post-hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.