Figure 4.

Correction of histological abnormalities in treated mice

Shown is histological analysis of the brain, heart, liver, spleen, lungs, and kidneys 16 weeks after injection by H&E staining. Scale bars: 20 μm; hippocampus, 40 μm. Black arrows indicate vacuolation of Purkinje neurons and hippocampal pyramidal neurons or foamy macrophages in the heart, liver, spleen, lungs, and kidneys because of GAG accumulation. H&E staining of treated mice was obtained from high-dose groups. For low-dose groups, see Figure S6.