Fig. 1. Non-B DNA motifs at splicing junctions.

A Distribution of non-B DNA motifs relative to splice sites. Seven non-B DNA motifs are shown, namely direct repeats (DRs), G-quadruplexes (G4s), H-DNA, inverted repeats (IRs), mirror repeats (MRs), short tandem repeats (STRs) and Z-DNA. B Distance between nearest G4 motif/G4-seq peak and a splice site separately for 3′/5′ splice sites. C Venn diagrams for the occurrences of G4s within 100 nt of the 3′ss (upstream) and 5′ss (downstream) using the consensus G4 motif, the K⁺ treatment G4-seq derived DNA G4 peaks and the PDS treatment G4-seq derived DNA G4 peaks⁵¹ and reporting the overlapping G4s between them. D Distribution of G4 motifs relative to cassette exons, alternative acceptor and alternative donor. The orange colour in the schematic represents the alternatively included exonic part, corresponding to each type of alternative splicing event.