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. 2022 May 3;13:2404. doi: 10.1038/s41467-022-30071-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Density of G4 motifs in a 100 nt window on each side across all upstream (3’ss) and downstream (5’ss) splice sites of each species, measured as mean values. Error bars indicate standard deviation from 1000-fold bootstrapping with replacement. B Enrichment of G4 motifs at splice sites for seven vertebrate species, using the consensus G4 motif. C Enrichment of G4-seq derived DNA G4s at splicing sites at 100 nt splicing site windows in PDS and K⁺ treatments, measured as mean values. Error bars indicate standard deviation from 1000-fold bootstrapping with replacement. D Enrichment of DNA G4s at: upstream (3’ss) and downstream (5’ss) splice sites across six species for PDS and K⁺ treatments.