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. 2022 May 4;7:147. doi: 10.1038/s41392-022-00945-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Pearson correlation analysis was performed between MrgprF mRNA expression and drug IC₅₀ using the GDSC database. Blue bubbles: negative correlations; Red bubbles: positive correlations, the deeper the color is, the higher the correlation is. Bubble size is positively associated with the FDR significance. Black outline border indicates FDR < 0.05. b Cell viabilities following treatment with the indicated concentration of AMG 706 for 48 h were examined by sulforhodamine B (SRB) staining. The IC₅₀ for each cell line is indicated. c AMG 706 (15 and 30 μM) 24 h treatment increased MrgprF expressions examined by Real-time RT-PCR (top) and immunoblot (bottom) in A375 cells. d, e AMG 706 (15 μM) treatment in A375 cells decreased cell proliferation as examined by BrdU incorporation (d) and colony formation (e) assays. Quantification data are shown. f, g AMG 706 (15 μM) treatment in A375 cells promoted G0/G1 phase arrested cell population as examined by FACS (f) and immunoblot (g). Indicated cell lysates were probed with the indicated antibodies. Quantification data is shown. h, i AMG 706 (15 μM) treatment in A375 cells suppressed cell migration as examined by trans-well (h) and immunoblot (i). Indicated cell lysates were probed with indicated antibodies. Quantification data is shown. Scale bar = 50 μm. j Immunofluorescence staining of Phalloidine in A375 treated with DMSO or AMG 706 (15 μM). White head arrows pointing to the pseudopodia like structure. Scale bar = 25 μm. k Schematic view of xenograft mouse model treated by indicated drugs. (l-n) AMG 706 inhibited xenograft tumor growth in vivo with AMG 706 (7.5 mg/kg) or/and DDP (7 mg/kg) treatments. Representative xenograft tumor images (l), tumor volumes (m, data are presented as mean ± SEM) and tumor masses (n) are shown for the indicated mouse groups. A375 cells were used. o, p Representative IHC staining of MrgprF, Ki67, CC3, and p-Akt in indicated xenograft tumors. p Quantification data for (o). Scale bar = 50 μm. Quantified results for all the immunoblots are indicated below, which are normalized to the β-actin signal, compared to reciprocal control. Bars are the mean value ± SD. *P < 0.05, **P < 0.01, ***P < 0.001