Fig. 6. AMG 706 treatment inhibited the metastatic tumor progression.

a Schematic view of the lung metastasis mouse model treated by indicated drugs. AMG706 and vehicle were injected daily starting from day 3 after B16 injection. b, c Representative lung tissues were shown (b), and the metastatic tumor numbers in lung tissues were quantified (c). d, e Representative images of the H&E and IHC staining using the indicated metastatic lung tumors. Antibodies for MrgprF, E-cadherin, N-cadherin, and Ki67 were used. e Quantification data for (d). Scale bar = 50 μm. E-cad: E-cadherin; N-cad: N-cadherin. f Representative images of the H&E staining of the vital organs in mice (Heart, Liver, Spleen, Kidney, and Brain), with or without AMG 706 treatment. g A model deciphering how MrgprF inhibited melanoma progression. Bars are the mean value ± SD. *P < 0.05, **P < 0.01, ***P < 0.001