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. 2022 Mar 28;12(7):3131–3149. doi: 10.7150/thno.69217

Figure 5.

Figure 5

Olig2 interacted with PPARγ and participated in phospholipogenesis involved in OL differentiation partially through SMARCA4 (Brg1) expression. A Flow cytometric analysis of the effect of pharmacological inhibition of PPARγ or CEPT1 knockdown on Olig2-OPC differentiation, and O4+ cells were determined at d21 of differentiation. B Quantification of O4+ cells at d21 of differentiation (*** p < 0.001, by a two-tailed Student's t test). C Immunostaining analysis of MBP expression to explore the effect of pharmacological inhibition of PPARγ or CEPT1 knockdown on Olig2-OPC differentiation at d21 of differentiation (scale bar, 50 μm). D Quantification of MBP+ cells at d21 of differentiation (*** p < 0.001, by a two-tailed Student's t test). E PPI networks among the main differentially expressed genes in control-OPCs and Olig2-OPCs indicated that overexpression of Olig2 might promote OL differentiation partially through the SMARCA4/Brg1-PPARG-CEPT1 signaling axis. F qPCR results indicated that the mRNA expression level of SAMRCA4/Brg1 was significantly upregulated in Olig2-OPCs (** p < 0.01, by a two-tailed Student's t test). The graphs represent the individual data points and the mean ± SEM of three independent experiments. Immunofluorescence images are representative of n = 3 biological replicates.