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. 2022 Apr 24;12(7):3553–3573. doi: 10.7150/thno.68895

Figure 3.

Figure 3

M2-EVs treatment promoted white matter structural remodeling and function repair in mice after 28 days of tMCAO. (A) Representative images and quantitative data of demyelinated axon (SMI32+, red) relative to myelin (MBP+, green) in the corpus callosum, striatum and cortex of the M2-EVs treated, the M0-EVs treated, the PBS treated and sham group after 28 days of tMCAO (n = 4). Scale bar = 100 µm. (B) Representative low and high magnification TEM images of myelinated axons in the striatum of each group after 28 days of tMCAO. Scale bar = 0.5 µm. (C) The g-ratio of myelinated axons with different axon diameter in each group (n = 3, 200-400 axons per mouse). (D) Scatter plots of g-ratio with respect to different axon diameter in each group. (E-F) Representative 350-μm-thick coronal brain sections at bregma +0.51 (E) mm and bregma -1.59 mm (F), and the N1 (left panel) and N2 (right panel) amplitude of the corpus callosum CAPs in each brain section after 28 days of tMCAO (n = 5-6). Data are mean ± SD, */#/$/&, p < 0.05. **/$$/&&, p < 0.01, ***/###, p < 0.005; *, M2-EVs group vs. PBS group; #, M0-EVs group vs. PBS group; $, M2-EVs group vs. M0-EVs group; &, PBS group vs. Sham.