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. 2022 Apr 24;12(7):3438–3455. doi: 10.7150/thno.61433

Figure 3.

Figure 3

CRAMP protects against C. rodentium-accelerated STZ-induced diabetes. (A) Animal protocol. Three-week-old WT and Cnlp-/- mice were infected with the C. rodentium weekly along with the corresponding intervention as previously described from 3 to 9 weeks of age (n = 12). Infected mice were then injected with a low-dose of STZ by intraperitoneal injection for 5 consecutive days to induce T1D. All the mice received two injections of CRAMP (100 µg, twice a week) or saline by intraperitoneal injection twice a week. Mice were monitored for the glucose from the tail vein after 6 h fasting. (B) Insulitis and insulitis score in STZ-induced diabetic mice, n = 6. Scale bar: 50 µm. (C) Blood glucose was tested after 6 h fasting in STZ-induced diabetic mice, n = 12. (D) STZ-induced Cnlp-/- and WT diabetic mice were considered to have severe diabetes when glycemia was > 300 mg.dL-1 (16.6 mmol.L-1) after two consecutive determinations. (E) Glucose tolerance tests were performed after 18 h fasting at 3 weeks after STZ injection, n = 6. (F) Serum and pancreatic insulin levels were detected in STZ-induced diabetic mouse model at 3 weeks after STZ injection, n = 8. Data are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.