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. 2022 Apr 4;12(7):3196–3216. doi: 10.7150/thno.71029

Figure 7.

Figure 7

Knockdown of neuronal PTRF protects brain against cerebral I/R injury. (A) Western blot was used to analyze the expression levels of Caspase3, Bax, and Bcl-2 in AAV-shRNA transfected primary neuronal cells under OGD/R. (B) The experimental protocol used in mice transfected with AAV-shRNA or AAV-Scra after I/R injury. (C) The corner turn test was analyzed using the laterality index (n = 6). (D) Neurological deficit was assessed throughout recovery at 24 h and 72 h post-cerebral I/R injury (n = 8). (E-F) TTC stained brain slices showing infarct volume (white), and the infarct volume was measured in the mice transfected with AAV-shRNA or AAV-Scramble after cerebral I/R injury (n = 3). (G) Survival rates at 3 d in the mice transfected with AAV-shRNA or AAV-Scramble after cerebral I/R injury (n = 25). (H) The expression levels of Caspase3, Bax, and Bcl-2 were measured by Western blot analysis in the ipsilateral penumbras from AAV-shRNA or AAV-Scramble transfected mice after I/R injury. (I) The corner turn test was analyzed using the laterality index at 15 d post-cerebral I/R injury in mice (number of left turns -number of right turns)/10 (n = 6). (J) Daily neurological deficit score was assessed over the 15 recovery days (n = 6). (K) Mouse survival rates at 15 d post-cerebral I/R injury in mice (n = 25).