TABLE 2.

Nucleotide sequence of PCR primers used to amplify the gyrA gene (C 94V, C 121V, C 316R, C 358R, CdgaV, and CdgaR) and 16S rDNA (609V and 699R) of C. difficile

Primer	Sequence
C 94V	5′-CGTGCACT(G/C/T)CC(A/C/G)GATGT-3′
C 121V	5′-CTTAAGCC(A/G/T)GTTCAT(A/C)G-3′
C 316R	5′-GAACCAAAGTT(A/T)CC(G/T/A)TG-3′
C 358R	5′-GCTTCTGT(A/G)TA(A/T)C(T/G)CAT-3′
CdgaV	5′-TTTAAAGCCAGTTCATAG-3′
CdgaR	5′-GAACCAAAGTTACCATG-3′
609V	5′-GGATTAGATACCC(C/G/T)(A/G/T)GTA-3′
699R	5′-(A/T/G)GGGTTGCGCTCGTT(A/G)C-3′