Figure 1.

Adipose tissue-specific Pgam1 deletion has differing effects on BAT and WAT

(A) Relative transcription level for Pgam1 and Pgam2 of whole body organs from 12-weeks-old C57BL/6N mice (each n = 4).

(B) Transcripts for Pgam1 of BAT (left panel) and gonadal WAT (gWAT) (right panel) from Adipo-Pgam1 knockout (Adipo-Pgam1 KO) and their littermate WT (Cont) mice (BAT; n = 4, 4, gWAT; n = 4, 4).

(C) Left; Western blot analysis for Pgam1 in BAT (left) and gWAT (right) prepared in (B). Actin was used as loading control. Right; Pgam enzymatic activity of BAT (left) and gWAT (right) prepared in (B) (BAT; n = 9, 9, gWAT; n = 9, 9).

(D) Body weight of mice prepared in (B) (n = 19, 16).

(E) Food intake of mice prepared in (B) (n = 11, 8).

(F) Visceral fat weight of mice prepared in (B) analyzed with CT scan (n = 12, 9).

(G) BAT weight of mice prepared in (B) (n = 19, 16).

(H) gWAT weight of mice prepared in (B) (n = 19, 16).

(I) Oxygen consumption (VO₂)(n = 16, 10), CO₂ emission (VCO₂)(n = 16, 10), respiratory exchange ratio (RER)(n = 16, 10) and energy expenditure (EE)(n = 16, 10) of mice prepared in (B).

(J) Insulin tolerance test (ITT) (left) (n = 9, 4), glucose tolerance test (GTT) (middle) (n = 9, 4) and plasma insulin concentration during GTT (right) (n = 7, 3) of mice prepared in (B).

(K) Acute cold tolerance test in mice prepared in mice prepared in (B) (n = 16, 13).

(L) Hematoxylin and eosin (HE) staining of gWAT (upper panel), BAT (middle panel) and iWAT (lower panel) from mice prepared in (B). Scale bar = 100μm. Data were analyzed by the 2-tailed Student’s t test (B–I), or repeated measures followed by Tukey’s multiple comparison test (J and K). ∗p < 0.05, ∗∗p < 0.01. Values represent the mean ± SEM NS = not significant. See also Figure S1.