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. 2022 Apr 18;25(5):104268. doi: 10.1016/j.isci.2022.104268

Figure 3.

Figure 3

Adipo-Pgam1 KO mice show whitening of BAT and beiging of WAT

(A) Immunofluorescent staining showing Ucp1 (green) and Cpt1b (red) in BAT (left) and gWAT (right) from Adipo-Pgam1 KO and their littermate WT mice. Scale bar = 100 μm. The graphs on the right display the Ucp1-or Cpt1b-positive area (%) (each n = 4, 4).

(B) Transcripts for Ucp1 and Cpt1b of BAT (left) and gWAT (right) from mice prepared in (A) (BAT; Ucp1 n = 8, 4, Cpt1b n = 6, 5, gWAT; Ucp1 n = 8, 4, Cpt1b n = 6, 5).

(C) Transmission electron microscopy analyzing BAT (left) and gWAT (right) from mice prepared in (A). Scale bar = 5μm for low magnification and 500 nm for high magnification in BAT and 2μm in gWAT.

(D) Quantification of TUNEL positive cells of BAT (left) and gWAT (right) from mice prepared in (A) (n = 4,4).

(E) Norepinephrine level in the circulation (left) and gWAT (right) from mice prepared in (A). (Plasma; n = 9, 4, gWAT; n = 6, 4). Data were analyzed by the 2-tailed Student’s t test (A, B, D, and E). ∗p < 0.05, ∗∗p < 0.01. Values represent the mean ± SEM NS = not significant. See also Figures S4–S6.