Figure 4.

Metabolic phenotypes of BAT-specific Pgam1 KO mice

(A) Transcripts for Pgam1 of BAT (left graph) and gWAT (right graph) from BAT-Pgam1 knockout (BAT-Pgam1 KO) and their littermate WT (Cont) mice (BAT; n = 7, 7, gWAT; n = 7, 7). Western blot analysis showing down-regulation of Pgam1 in BAT from BAT-Pgam1 KO mice.

(B) Body weight of mice prepared in (A) (n = 12, 12).

(C) Food intake of mice prepared in (A) (n = 7, 7). (D) Body weight-adjusted BAT (n = 12, 12), gWAT (n = 11, 12) and inguinal WAT (iWAT) (n = 11, 12) weight of mice prepared in (A).

(E) Acute cold tolerance test in mice prepared in mice prepared in (A) (n = 12, 12).

(F) Insulin tolerance test (ITT) (left) (n = 12, 13) and glucose tolerance test (GTT) (right) (n = 12, 13) of mice prepared in (A).

(G) Oxygen consumption (VO₂)(n = 7, 7), CO₂ emission (VCO₂)(n = 7, 7), respiratory exchange ratio (RER)(n = 7, 7) and energy expenditure (EE)(n = 7, 7) of mice prepared in (A). Data were analyzed by the 2-tailed Student’s t test (A–D and G), or repeated measures followed by Tukey’s multiple comparison test (E and F). ∗p < 0.05, ∗∗p < 0.01. Values represent the mean ± SEM NS = not significant.