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. 2022 Apr 18;25(5):104268. doi: 10.1016/j.isci.2022.104268

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© 2022 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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BAT-specific Pgam1 deletion does not promote beiging of WAT

(A) Hematoxylin and eosin (HE) staining of gWAT (upper panel) and BAT (lower panel) from BAT-Pgam1 knockout (BAT-Pgam1 KO) and their littermate WT (Cont) mice. Scale bar = 100μm.

(B) Transmission electron microscopy analyzing BAT (left) and gWAT (right) from mice prepared in (A). Scale bar = 5μm for low magnification and 2μm for high magnification in BAT and 2μm in gWAT.

(C) Immunofluorescent staining showing Ucp1 (green) and Cpt1b (red) in BAT (left) and gWAT (right) from mice prepared in (A). Scale bar = 100 μm. The lower graphs display the Ucp1-or Cpt1b-positive area (%) (each n = 4, 4).

(D) Transcripts for Ucp1 and Cpt1b of BAT (left) and gWAT (right) from mice prepared in (A) (each n = 7, 7). Data were analyzed by the 2-tailed Student’s t test (C and D). ∗p < 0.05, ∗∗p < 0.01. Values represent the mean ± SEM NS = not significant.