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. 2022 Apr 18;25(5):104268. doi: 10.1016/j.isci.2022.104268

Figure 6.

Figure 6

Pgam1 deletion affects BCAA metabolism

(A) Tissue weight-adjusted amino acid levels (nmol/g) in gWAT from Adipo-Pgam1 KO and their littermate WT mice (n = 5, 5).

(B) Western blot analysis for mTOR in gWAT prepared in (A). Actin was used as loading control. Right graphs show the quantification of p-mTOR/Actin ratio (n = 7,7).

(C) Western blot analysis for p62 in gWAT prepared in (A). Actin was used as loading control. Right graphs show the quantification of p62 expression (n = 7,6). Data were analyzed by the 2-tailed Student’s t test (A–C). ∗p < 0.05, ∗∗p < 0.01. Values represent the mean ± SEM NS = not significant. See also Figures S7 and S8.