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Chromatogram peaks of flucloxacillin at LLOQ (2 mg/L) (a) and SIL-IS (m/z 460 → 160) at concentration of 7 mg/L (b). Average peak intensity of the SIL-IS was approximately 50% higher than the LLOQ (Mean ± SD, 69939 ± 6451 cps (counts per second) (FLX); 104595 ± 8396 cps (SIL-IS), (n = 3)).