Table 2.
Morphological identity and developmental stage of the parasites found per host species, and sequence similarity scores obtained by BLAST (Basic Local Alignment Search Tool) for the present ascaridoid ITS, cox2 and 28S gene sequences, with the conclusion for parasite species identity.
| Host species | Morphology | Gene | BLAST (N, bp) | Acc. nr. | Parasite species |
|---|---|---|---|---|---|
| E. muticus | 1 L3 Anisakis sp. larval type I | NA | |||
| 1 L3 H. amoyense/H. zhoushanense | NA | ||||
| H. nehereus | 16 L3 H. amoyense/H. zhoushanense | ITS (n = 13) | 100% H. amoyense (381 to 804 bp) | KP252131 | H. amoyense |
| cox2 (n = 4) | 97.01–97.97% H. amoyense (356 to 583 bp) | MF120253 | |||
| 28S (n = 3) | 100% H. amoyense (724 to 725 bp) | MF094276 | |||
| S. fimbriata | 1 L3 H. amoyense/H. zhoushanense | ITS (n = 1) | 100% H. amoyense (292 bp) | KP252131 | H. amoyense |
| cox2 (n = 1) | 97.35% H. amoyense (432 bp) | MF120253 | |||
| 28S (n = 1) | 100% H. amoyense (724 bp) | MF094276 | |||
| S. crumenophthalmus | 3 L3 Anisakis sp. larval type I | ITS (n = 1) | 100% A. typica (897 bp) | JQ912690 | A. typica |
| cox2 (n = 2) | 97.74–99.30% A. typica (574 bp) | JQ859931 and JQ859923 | |||
| 28S (n = 2) | 100% A. typica (731 and 751 bp) | KX098562 | |||
| T. lepturus | 4 L3 Anisakis sp. larval type I | ITS (n = 2) | 100% A. typica (720 to 814 bp) | JQ912690 | A. typica |
| cox2 (n = 2) | 98.74–99.48% A. typica (578 bp) | KC928267 | |||
| 87 L3 + 2 L4 H. amoyense/H. zhoushanense | ITS (n = 4) | 99.87–100% H. amoyense (743 to 809 bp) | KP252131 | H. amoyense | |
| cox2 (n = 6) | 97.01–97.60% H. amoyense (462 to 587 bp) | MF120253 | |||
| 28S (n = 5) | 100% H. amoyense (711 to 725 bp) | MF094276 | |||
| U. duvaucelii | 1 L3 Lappetascaris sp. type A | ITS (n = 1) | 100% Hysterothylacium sp. or Lappetascaris sp. (874 bp) | MT365537 or MW750364 | Lappetascaris sp. |
| cox2 (n = 1) | 98.78% Lappetascaris sp. (575 bp) | MW775335 | |||
| 28S (n = 1) | Matches <96.5% |
Morphology: number of ascaridoids identified to species or larval type and developmental stage (i.e. L3 = third larval stage, L4 = fourth larval stage); Gene: ribosomal (ITS or 28S) or mitochondrial (cox2) DNA sequences; BLAST: blast results (percent identity (%) and species identity), bp: number of base pairs; Acc. Nr.: Accession number of the highest match from published deposited samples; Parasite species = conclusion for species identity. NA: not available.