Figure 5.
DARPin F10 impairs cellular granules formation
(A) Aggresome formation was induced with MG132 in indicated cells (−/+ dTAG pre-treatment). Aggresomes were detected by staining for HDAC6 and Ub; DAPI was used to stain the nucleus. Scale bar, 20 μm.
(B) Quantification of aggresome formation by Ub signal (n = 3). The graph shows the percentage of aggresome-positive cells; each data point represents the percentage obtained in a randomly chosen micrograph containing ca. 50 cells. Statistical analysis was done by one-way ANOVA; p values refer to significant (< 0.05) differences between samples. Data are represented as mean ± SD.
(C) SGs were induced with NaAsO2 in indicated cells (−/+ dTAG pre-treatment); SGs were visualized and quantified by staining for G3BP1. Representative pictures are shown. Scale bar, 20 μm.
(D) Quantification of the number of SGs per cell area. Each data point represents the number of SGs per cell area in a sample containing one or a few (ca. 2 to 5) cells. Data were collected in five independent experiments (n = 5). Statistical analysis as in (B).