REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
OCT4 mouse monoclonal antibody	Santa Cruz Biotechnology	sc-5279
OCT4 rabbit monoclonal antibody	Abcam	Ab200834
Anti-Cardiac Troponin T-FITC	Miltenyi Biotec	130-119-575
REA Control(I)-FITC	Miltenyi Biotec	130-118-354
α-actinin	Sigma-Aldrich	A7811
βIII tubulin	Cell Signaling Technology	5666
Donkey anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 594	Thermo Fisher Scientific	A21203
Donkey anti-mouse IgG (H+L) highly cross-adsorbed secondary, Alexa Fluor 488	Thermo Fisher Scientific	A21202
Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary, Alexa Fluor 546	Thermo Fisher Scientific	A11030
Goat anti-rabbit IgG (H+L) cross-adsorbed secondary, Alexa Fluor 488	Thermo Fisher Scientific	A11008
Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary, Alexa Fluor 546	Thermo Fisher Scientific	A10040
Chemicals, peptides, and recombinant proteins
DMEM/F12 GlutaMAX	Thermo Fisher Scientific	10565-018
StemFit AS103C	Ajinomoto	N/A
StemFit AK02N	Ajinomoto	AK02N
StemFit AS501	Ajinomoto	N/A
RPMI 1640	FUJIFILM Wako Pure Chemical	189-02025
MEMα	Thermo Fisher Scientific	12571-048
Opti-MEM™ I Reduced Serum Medium	Thermo Fisher Scientific	31985070
Bovine Albumin Fraction V (7.5% solution)	Thermo Fisher Scientific	15260037
D-PBS	FUJIFILM Wako Pure Chemical	045-29795)
Y-27632	FUJIFILM Wako Pure Chemical	034-24024
TrypLE Select	Thermo Fisher Scientific	12563-011
Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix	Corning	354230
iMatrix-221	Matrixome	892 061
iMatrix-511	Matrixome	892 011
B-27™ supplement, minus insulin	Thermo Fisher Scientific	A1895601
Fetal bovine serum chile, USDA approved	Biowest	S1560-500
2.5 g/L Trypsin EDTA	NACALAI TESQUE	35554-64
Lipofectamine™ Messenger MAX™	Thermo Fisher Scientific	LMRNA001
Dimethyl sulfoxide (DMSO)	Sigma-Aldrich	D2650-100ML
Orlistat	Sigma-Aldrich	O4139-25MG
Hoechst 33342	Thermo Fisher Scientific	H3570
DAPI	Dojindo	D523
Experimental models: Cell lines
253G4	Center for iPS Cell Research and Application (CiRA), Kyoto University	N/A
201B7	CiRA, Kyoto University	N/A
H9	WiCELL	WA09
FfI14	CiRA, Kyoto University	https://www.cira-foundation.or.jp/e/project/homozygous.html
Oligonucleotides
NEUROG2	(Zhou et al., 2014)	N/A
Recombinant DNA
NEUROG2 and 3′UTR DNA	(Goparaju et al., 2017)	N/A
Software and algorithms
ImageJ	National Institutes of Health	https://imagej.nih.gov/ij/
cellSens	Olympus	https://www.olympus-lifescience.com/en/software/cellsens/
Other
4% Paraformaldehyde	Muto Pure Chemicals	33111
Triton X-100	Sigma-Aldrich	T9284
ImmunoBlock	KAC	CTKN001
LIVE/DEAD® Viability/Cytotoxicity Kit for Mammalian Cells	Thermo Fisher Scientific	L3224
Leukocyte Alkaline Phosphatase Kit	Sigma-Aldrich	86R-1KT