Figure 2.

Effect of the NF-κB inhibitor TPCA-1 on IL-1β-mediated IL-8 mRNA expression. Canine cardiac fibroblasts were pretreated with or without TPCA-1 (10 µM) for 1 h and subsequently stimulated with or without IL-1β (100 pM) for 24 h. After stimulation, IL-8 mRNA expression levels (A) and the release of IL-8 (B) were determined. HRPT1 was used as an internal standard and the expression levels of IL-8 mRNA in IL-1β-stimulated fibroblasts were compared with the expression at 0 h. Results have been represented as mean ± standard error (SE) from biological triplicates. *P <0.05.