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. 2022 Apr 20;13:863309. doi: 10.3389/fimmu.2022.863309

Figure 3.

Figure 3

IL-1β-mediated activation of NF-κB. (A) Representative western blotting results of levels of total IκBα (t-IκBα), phosphorylated p65 (p-p65), total p65 (t-p65), phosphorylated p105 (p-p105), and total p105 (t-p105) in canine cardiac fibroblasts treated with IL-1β (100 pM). β-actin was used as an internal standard. Time-dependent changes of levels of t-IκBα, p-p65 and p-p105 were observed. Relative levels of t-IκBα (B), [p-p65]/[t-p65] (C), and [p-p105]/[t-p105] (D) in IL-1β-stimulated fibroblasts compared to the levels at 0h. Results have been represented as mean ± SE from biological triplicates. *P <0.05.