Skip to main content
. 2022 Apr 20;13:863309. doi: 10.3389/fimmu.2022.863309

Figure 5.

Figure 5

Transfection with p65 siRNA attenuated IL-1β-mediated IL-8 mRNA expression in canine cardiac fibroblasts but not that with p105 siRNA. In canine cardiac fibroblasts transfected with p65, p105, and scrambled siRNAs, expression of t-p65, t-105, and β-actin was detected by western blotting. The expression of p65 or p105 was reduced in fibroblasts transfected with p65 or p105 siRNA, respectively, but not in fibroblasts transfected with scrambled siRNA. β-actin was used as an internal standard. Representative results (A) and relative density of protein expression of t-p65 (B) or t-p105 (C) in siRNA-transfected fibroblasts compared with those in scrambled siRNA-transfected fibroblasts are depicted. (D) Cardiac fibroblasts transfected with p65, p105 and scrambled siRNAs were incubated with or without IL-1β (100 pM) for 24 h After the incubation, IL-8 mRNA expression was determined. HRPT1 was used as an internal standard. Transfection with p65 siRNA resulted in reduction of IL-1β-mediated IL-8 mRNA expression, while p105 and scrambled siRNA-transfection did not. Results have been represented as mean ± standard error (SE) from biological triplicates. *P <0.05; N.S, Not Significant.