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Experimental validation of core predictors. (A) Coculture system of THP1 and Caco2 cells. (B) Validated Western blotting results for TLR2 overexpression and knockdown. (C) CCK8 results for infliximab and LPS in Caco2 cells. (D) RT-qPCR results for cocultured Caco2 cells after infliximab treatment in the context of LPS-induced inflammation (one-way ANOVA, *p < 0.05, **p < 0.01, and ***p < 0.001).
