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. 2022 Apr 20;13:851242. doi: 10.3389/fphar.2022.851242

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Copyright © 2022 Rezaei, Rezaei, Karimifard, Mahmoudi Beram, Dakkali, Heydari, Afshari-Behbahanizadeh, Mostafavi, Bokov, Ansari, Farasati Far, Akbarzadeh and Chaiyasut.

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(A) Percentage cell viability of different dilutions of niosomes on non-malignant MCF10A cells. (B) The effects of 5-FU, Nio/5-FU, Nio/5-FU/PEG, Nio/5-FU/HA and Nio/5-FU/FA on the viability of MCF7 cells. (C) The effects of 5-FU, Nio/5-FU, Nio/5-FU/PEG, Nio/5-FU/HA and Nio/5-FU/FA on the viability of 4T1 cells. (D) Half-maximum inhibitory concentration (IC₅₀) values after 48 h of exposure of MCF7 breast cancer cells to 5-FU, Nio/5-FU, Nio/5-FU/PEG, Nio/5-FU/HA and Nio/5-FU/FA. (E) IC₅₀ values after 48 h treatment of malignant 4T1 cells to 5-FU, Nio/5-FU, Nio/5-FU/PEG, Nio/5-FU/HA and Nio/5-FU/FA. Data represent means ± standard deviations (n = 3). For all charts, ***: p < 0.001; **: p < 0.01; *: p < 0.05.