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. 2022 Apr 20;13:887681. doi: 10.3389/fimmu.2022.887681

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Copyright © 2022 Shao, Cornwell, Xu, Kirchhoff, Saasoud, Lu, Jiang, Criner, Wang, Rogers and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SM + M treatment decreased CD4+CD25^highCD127^low Treg cells in the lung and lymph nodes. (A) Schematic representation of stimulations in four groups of male mice: 1) non-stimulation controls (NS); 2) smoking (SM); 3) morphine (M); and 4) smoking plus morphine (SM + M). B-D. The flow cytometry detections were performed for analyzing the populations of CD45 (lung only), CD3, CD4, CD8, CD25^highCD127^low Treg in (B) lung (n = 4), (C) spleen, and (D) lymph node (LN) (n = 8). Bar graphs indicate the percentage of each subset in its parent population. Percentage of CD25^highCD127^low Treg in all cells from lung, spleen and LN were calculated and showed in the last row. (T-test, *p < 0.05, **p < 0.01, ***p < 0.001, * in black represents the difference between SM, M or SM+M and NS control, * in red represents the difference between SM+M and SM or M).