Skip to main content
. 2022 Apr 20;13:887681. doi: 10.3389/fimmu.2022.887681

Figure 2.

Figure 2

Morphine (M) is stronger than cigarette smoke (SM) in re-shaping Treg cell transcriptomes and SM + M have synergy in modulating Treg cell transcriptomes. (A) Volcano plots illustrate log 10 (p-value) in relation to the log 2 (fold change) for the treatments versus control (n = 2). The differentially expressed genes with -log10 (p. value) >1.3 and |log2FC| >1 are displayed in red. Cytokines, chemokines and receptors with transcriptomic changes are labeled. (B) Bar graph show the level of transcripts of Foxp3 and cofactors, Treg effector molecules, and immune checkpoint molecules in Treg from four groups. (C) Bar graph show the level of transcripts of 15 nicotinic receptors and 4 opioid receptors in Treg from four groups. TPM, Transcripts per Million mapped reads (T test, *p < 0.05, **p < 0.01, ***p < 0.001, * in black represents the difference between SM, M or SM+M and NS control, * in red represents the difference between SM+M and SM or M).