Mutant KATPchannels are not trapped in the ER.A, top, representative immunostaining results show the colocalization of HA and KDEL in transfected INS-1 treated with 10 μM Carb treatment for 16 h. B, bottom, the bar graph shows the mean fluorescence intensity of colocalized HA (green) and KDEL (red) signals. Data are presented as the mean ± SEM; n = 5 per group; the scale bar represents 10 μm. Carb, carbamazepine; ER, endoplasmic reticulum; HA, hemagglutinin; KATP, ATP-sensitive potassium channel.