Figure 7.

Autophagy blocker and proteasome inhibitor increase the levels of mature SUR1 in mutant K_ATPchannels.A, representative immunoblotting images show the levels of SUR1 in INS-1 cells expressing WT (HA-SUR1/Kir6.2) or mutant (HA-SUR1/Kir6.2-A28V) K_ATP channels, after treatment with 3MA, MG132, and chloroquine (CQ). Quantification of upper (mature) SUR1 is shown in (B) and lower SUR1 is shown in (C) Data are presented as the mean ± SEM; n = 3 per group; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005 compared with DMSO-treated cells in each corresponding group; differences were evaluated using two-way ANOVA and Dunnett's post hoc test. DMSO, dimethyl sulfoxide; HA, hemagglutinin; K_ATP, ATP-sensitive potassium channel; SUR1, sulfonylurea receptor 1.