Figure 7.

A_2AR^−/−mice have decreased CtsD protein expression in IPMΦ cells. Levels of different CtsD forms (PF, IF, and HC) and β-actin were measured in protein extracts from WT and A_2AR^−/− mice in IPMΦ cells, using a CtsD-specific polyclonal antibody. Sample loading was normalized to β-actin. Statistical analyses of the relative expression of CtsD total proteins and HC form were based on three independent samples evaluated by independent t test. Data are presented as mean ± SD. ∗p < 0.0145, ∗∗p < 0.0074 versus control (A_2AR^+/+) cells. A_2AR, adenosine A_2A receptor; CtsD, cathepsin D; HC, heavy chain; IF, intermediate form; IPMΦ, mouse peritoneal macrophage cell; PF, proform.