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. 2022 Apr 1;298(5):101887. doi: 10.1016/j.jbc.2022.101887

Figure 3.

Figure 3

PALMD regulates the glycolytic and inflammatory gene expression profile in hVICs. hVICs were transfected with 20 nM siScrambled or siPALMD for 48 h. RNA-Seq–based transcriptomic profiling was performed. The RNA-seq data is deposited in NCBI Gene Omnibus (http://www.ncbi.nlm.nih.gov/geo/) with the accession number of GEO: GSE165524. A, volcano plot with green dots representing significantly downregulated protein-coding genes (n = 380) and red dots representing significantly upregulated protein-coding genes (n = 371) in PALMD-silencing and control hVICs. B, hierarchical clustering heat map of 751 protein-coding genes differentially expressed between PALMD-silencing and control hVICs (1.5 fold change, p < 0.05), n = 3. C, top ten upregulated genes and downregulated gene identified by RNA-Seq was validated using RT-qPCR, n = 6. D, correlation between RNA-Seq and RT-qPCR. Comparison of log2 fold change of 20 genes obtained by RNA-Seq and RT-qPCR. E, gene ontology and F, KEGG pathway analysis of the downregulated genes in PALMD-depleted hVICs identified by RNA-Seq. hVIC, human valve interstitial cell.