Figure 3.

Responding T cell signature. Frequencies of (a) CD4⁺ and (c) CD8⁺ T cells expressing IFNγ, IL2, and TNFα shown as % of the parent population. Polyfunctional signatures of both (b) CD4⁺ and (d) CD8⁺ T cells shown in representative pie charts. Heat maps were included, for each subset of T cell, to describe the relative amounts of each polyfunctional profile. Both heat maps show the percentage of CD4⁺ or CD8⁺ T cell in each category on a continuum from 0% (blue) to increasing % (red). Numbered and colour coded categories within each pie chart/heat map represent various T cell profiles of cytokine expression and are explained in the included legends. All data are presented as the mean ± SEM of net values (stimulated cells – unstimulated cells) from two independent experiments. (n=8). *p<0.05, **p<0.01 (two-way ANOVA).