Table 1.

Overview of the used strains with their respective measured minimum inhibitory concentration (MIC) and null-model likelihood of the corresponding predicted MICs (see main text).

wild-type straina (without TEM)	TEM amino acid substitution	strain name (as used here)	MIC for Cefotaximeb (µg ml−1)	null-model likelihood Lc (%)
MG1655 galK::SYFP2-CAT	none (TEM-1)	ancestor	0.08	>99.99
	G238S	single mutant	0.64	1.1
	E104 K/G238S	double mutant	10.24	<0.01
	E104 K/M182 T/G238S	triple mutant	81.92	<0.01

^aMIC of the wild-type strain for CTX = 0.04 µg ml⁻¹.

^bAs determined prior to the study by standard MIC assays (see Material and methods). Note that the true MIC lies between this value and half of this concentration (due to the 2-fold dilution during the setup).

^cAssuming that the inoculum establishes at the highest concentration at which growth is observed in the MIC assay (see the last section of Results and Discussion); values that show significant differences between the measured and predicted MICs are depicted in bold.