Table 3.

Sequences of gRNAs and the Cas9-repair templates used for Cas9-mediated gene editing

Name	Sequence 5′-3′
JA167 PBA1 (gRNA F)	CATATTTTGCTAGAGTAGAGgtttt
JA168 PBA1 (gRNA R)	CTCTACTCTAGCAAAATATGgatca
JA178 PBA1 2-12Δ (Cas9 repair)	ATATCATCGCACTACAGTAAAATTTTCATTTATAGCGATGAAACATCTGCTAGATCTCCCAGAGATTTCAAAAAACCTGC
JA179 PBA1 2-17Δ (Cas9 repair)	ATATCATCGCACTACAGTAAAATTTTCATTTATAGCGATGCTCCCAGAGATTTCAAAAAACCTGCAATCTTTAGAGTCT
JA218 PBA1 F139A (gRNA F)	GACGTTATTATTTTCTATGGgtttt
JA219 PBA1 F139A (gRNA R)	CCATAGAAAATAATAACGTCgatca
JA220 PBA1 F139A (Cas9 repair)	TTCATTTGGAAAGACGTTATTATTTTCTATGGAaGAGAATgctATTAGTATATCGCCGATTTTTGGTAACATGATAAGTA
JA221 PBA1 D172A (gRNA F)	TCCCCTGATATAATAGTAATgtttt
JA222 PBA1 D172A (gRNA F)	ATTACTATTATATCAGGGGAgatca
JA223 PBA1 D172A (Cas9 repair)	AGCCCAGTTCTCCCCaGAcATtATAGTtATTGGCACCTCTGcTAAAATCGCCAGCATGAAGGTAATGACGGAAAATGAAT

Abbreviations: F, forward; gRNA, guide RNA; R, reverse.