Table 3.

Single-molecule parameters of EGFP-HM19^FullLZ

ATP concentration	Step size	Dwell time (τ)	Run length (λ)	Velocity
	nm	s	μm	nm s−1
5 μM ATPa	NDb	ND	0.14 ± 0.06 (n = 34)	54.4 ± 4.8 (n = 34)
1 mM ATPa	ND	ND	0.18 ± 0.01 (n = 47)	95.1 ± 7.4 (n = 47)
2 μM ATPc	33.8 ± 1.3 (n = 91)	1.4 ± 0.1 (n = 46)	0.17 ± 0.01 (n = 41)	ND

Experiments were done on single actin filaments in the presence of the indicated ATP concentration (see the “Experimental procedures” section). Mean ± SEM was shown.

^aEGFP fluorescence was monitored.

^cQdot was used.

^bND, not determined.