Identifications of the differential abundance of genera in cecal microbiota (n = 6 per group). (A) Analysis by metagenomeSeq: the differential abundance of microbes in the cecum between groups was analyzed at the age of 4, 7, 9, and 11 weeks: * p<0.05 and ** p<0.01. (B) STAMP analysis at same different ages of time. The bar plot demonstrated the differential abundance of genera between the control and experimental groups at the age of 11 weeks. Only significant features with p<0.05 (Welch’s t-test) were shown. Significant increments of Bernesiella, Prevotellaceae_NK3B31_group, and Butyrucimonas (p<0.05) were noted with remarkable decrements of Bacteroides, Rikenellaceae_RC9_gut_group, and Succinatimonas (p<0.05) (C) linear discriminant analysis effect size (LEfSe) analysis with linear discriminant analysis (LDA) score >3.5 at the age of 11 weeks. Prevotellaceae_NK3B31_group, Rikenellaceae_RC9_gut_group, and Succinatimonas were identified as differentially significant and biological feature taxa between groups (p<0.05). Identifications of the differential abundance of genera in cecal microbiota (n = 6 per group). (A) Analysis by metagenomeSeq: the differential abundance of microbes in the cecum between groups was analyzed at the age of 4, 7, 9, and 11 weeks: * p<0.05 and ** p<0.01. (B) STAMP analysis at same different ages of time. The bar plot demonstrated the differential abundance of genera between the control and experimental groups at the age of 11 weeks. Only significant features with p<0.05 (Welch’s t-test) were shown. Significant increments of Bernesiella, Prevotellaceae_NK3B31_group, and Butyrucimonas (p<0.05) were noted with remarkable decrements of Bacteroides, Rikenellaceae_RC9_gut_group, and Succinatimonas (p<0.05) (C) linear discriminant analysis effect size (LEfSe) analysis with linear discriminant analysis (LDA) score >3.5 at the age of 11 weeks. Prevotellaceae_NK3B31_group, Rikenellaceae_RC9_gut_group, and Succinatimonas were identified as differentially significant and biological feature taxa between groups (p<0.05).