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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Biochemistry. 2021 Oct 27;60(44):3323–3336. doi: 10.1021/acs.biochem.1c00528

Figure 2. Multi-round nucleotide addition by RNAPII.

Figure 2.

(A) Experimental overview for preparation of labeled ECs on scaffold 1 and ligation to a 2.3 kb fragment of the E. coli rpoB gene (see Figure S2A for the sequence of the nucleic acid scaffold). (B) Time course of transcription by WT and H1085L RNAPII. Average nucleotide addition rates were determined from the change in the average transcript length (see Methods). H1085L RNAPII elongated at about two-thirds the rate of WT RNAPII (unpaired two-sample t-test P<0.0001). An uncropped image of the gel is shown in Figure S1B.