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. 2022 Mar 1;23(5):e53281. doi: 10.15252/embr.202153281

Figure EV3. Characterization of RGI expression in mature leaves, RGI3 expression in and phenotypic characterization of rgi5x pRGI3::RGI3 .

Figure EV3

  1. Semi‐quantitative reverse transcription PCR showing expression of RGI1‐RGI5 in 6‐week‐old leaves of WT and rgi5x. UBQ was used as a control. RGI3 and RGI4 show comparable expression levels. RGI5 shows weaker transcript levels and RGI1 and RGI2 expression was not detectable in mature leaves.
  2. Semi‐quantitative reverse transcription PCR showing expression of RGI3 in transgenic rgi5x pRGI3::RGI3 lines. UBQ was used as a control. Both lines of rgi5x pRGI3::RGI3 show RGI3 expression in mature leaves.
  3. Representative pictures taken 7 days after vertical germination of the indicated genotypes on ½ MS Agar plates.
  4. Quantification of main root length of seedlings shown in C. Shown is the mean of n = 12 biological replicates ± SD (one‐way ANOVA, Tukey post hoc test, a‐b P < 0.001). Expression of pRGI3::RGI3 does not complement the short root phenotype of the rgi5x mutant.

Data information: Similar results were obtained in three independent experiments.

Source data are available online for this figure.